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Intermittent "Your mailbox appears to be unavailable. Try to access it again in 10 seconds" in OWA 2010

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We have an "interesting" intermittent problem affecting one of our OWA (2010) installations.

We are in the middle of a migration from Exchange 2003 to Exchange 2010 (same forest / organisation).  2010 setup is 2 x CAS/HT, 2 x Mailbox in a DAG configuration, with all databases currently mounted on one host.  There are <20 users during this UAT phase using Exchange 2010.  All servers are SP2 Rollup 4.

Intermittently, when using OWA, the 2010 users are presented after login, the "Your mailbox appears to be unavailable.  Try to access it again in 10 seconds" message.  Pressing refresh in the browser instantly brings up the mailbox.  This happens on various browsers and client types.  After accessing the mailbox properly, the session continues normally without any perceived problems.  This has been ongoing for days.

All required Exchange services are running on all four servers (test-servicehealth comes back clean).

I have restarted the CAS/HT servers.  BPA seems clear of anything that might affect OWA.

Get-MailboxDatabaseCopyStatus shows that the databases are all mounted, and the copies are healthy.

Test-MapiConnectivity is successful to all databases.

Test-OutlookConnectivity is successful for all scenarios.

Test-OutlookWebServices is successful for all tests, with responses received from the correct services.

Event logs show nothing obvious, on CAS or Mailbox servers.

All I'm seeing is a long "time-taken" in the initial get request in the IIS logs on the CAS (over 20 seconds).

Any help or advice would be appreciated.

Many thanks in advance!

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